Molecular characterization of a clinical Haemophilus parainfluenzae isolate with cefotaxime resistance and decreased susceptibility to fluoroquinolones.

نویسندگان

  • Diego Faccone
  • Paula Lopez-Ruitti
  • Miryam Vazquez
  • Leonor Guerriero
  • Celeste Lucero
  • Paula Gagetti
  • Paola Ceriana
  • Alejandra Corso
چکیده

We report an H. parainfluenzae clinical isolate resistant to cefotaxime and with decreased susceptibility to ciprofloxacin recovered from a patient with cystic fibrosis. The isolate had elevated MICs of ampicillin (256mg/L), amoxicillin-clavulanate (8mg/L), cefuroxime (8mg/L) and cefotaxime (4mg/L), and showed a β-lactamase-producing amoxicillin-clavulanic acid-resistant (BLPACR) phenotype. A blaTEM-1 plus five amino acid substitutions in the PBP3 were found: Ser385Thr, Val511Ala, Ile519Val, Asn526Lys and Asp551Leu. MIC of ciprofloxacin was 0.5mg/L, and substitutions in gyrA (Ser84Tyr) and parC (Ser84Phe) genes were detected.

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عنوان ژورنال:
  • Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

دوره 44  شماره 

صفحات  -

تاریخ انتشار 2016